Redox imaging in the rice blast fungus Magnaporthe oryzae Plants respond to pathogen attack via a rapid burst of reactive oxygen species (ROS). However, ROS are also produced by fungal metabolism and are required for development of infection structures in Magnaporthe oryzae. To understand redox regulation in oryzae better, we measured the amount and redox…
Read more Redox imaging in the rice blast fungus Magnaporthe oryzae
We have pioneered network analysis of foraging basidiomycete fungi growing in unconstrained microcosms (Bebber et al. 2007a; Bebber et al. 2007b; Boddy et al. 2009; Boddy et al. 2010; Fricker et al. 2008a; Fricker et al. 2007; Fricker et al. 2008b; Heaton et al. 2010; Rotheray et al. 2008). We have shown using graph-theoretic analysis…
We developed a system for quantitative imaging of total cytoplasmic glutathione (GSH) in vivo following GST-catalysed conjugation to monochlorobimane (MCB) to give a fluorescent glutathione-bimane (GSB) adduct. This has allowed measurement of concentrations of GSH in defined cell types in intact tissues, to dissect the control of GSH synthesis and to analyse activities of GSH-based…
Under construction
Studies on radiolabelled amino acid transport in the dry rot fungus
Mark Fricker: Research 